PTC...abhi itni hi bani hai..

PTC

shabdaawali(terms to remeber):

haberlandt:father of tissue culture.
(icici par daddy ji(anupam kher of KKG)..with land ke papers)

white:successful long term culture of tomato roots
(1st hostel ke saamne,safed tomatoes)

morel and martin:culture of virus free dahlia through meristems
(Sac ke neeche.marty(maagascar?) making dalia)

miller and skoog:root:shoot,auxin:cytokinin
(tuck ke aage,uncle scrooge rate-io pooch re hain)

cocking:ezmyme,cell wall,protoplast
(nahi..main iska nahi bat sakta)


presentation:lab org and equipment

Cn=10^N X 9.1/d)^2.08

BSC1:collg waale cabinet with HEPA filter on exhaust.

BSC2:vertical waale,no volatle compund protection
dono jagah HEPA filter,input and exhaust.

type A:no vents,recirculated.
type b1:vented,30% exhausted,70% recirculated.
type b2:vented,all air exhausted.
type b3:70% exhausted,30% recirculated.

BSC 3:enclosed air tight,gloves,exhausted 2 hepa,

tetrazonium salts to red formazon:viable will be red.
evan's blue stain of dead
flourescein diacetate(FDA):green flourescein in viable.s