Sunday, November 23, 2008

ye hai biochem...kamino acids...

Urea cycle:
bicarbonate->carboxyphosphate->carbamic acid->carbomoyl phosphate
carbomoyl phosphate synthetase
(ac block)

actual cycle:

carbomyl phos+ornithine->citrulline
ornithine transcarbomylase

citrulene+asparate->argininosuccinate - fumerate->arginine
argininosuccinate sythetase
argininosuccinase

arginine+urea->ornithine
arginase

argininosuccinate->fumarate

deficiencies:

brain swelling due to excess of glutamine

argininisuccinase def.
excess agrinine is supplied.per molecule of arginine,2 nitrogen excreted.


carbomoyl phosphate synthase def,or ornithine transcarbomoylase def.accumulates as glycine

and glutamine
benzoate and phenylacetate


Benzoyl CoA->hippurate
(all out,photo,paper,plug pt.)
phenylacetate->phenyl acetyl CoA->phenyl acetyl glutamine



uricotelic:purine uric acid.


entry points:
pyruvate:SAC GWOT(har jyot)
serine,ala,cysteine, glycine,typtophan,threonine

OAA:aspartate,asparagine
DN
DoN

alpha keto glutarate:herp(es) q
HERP Q
histadine,glutamine,glutamate,arginine,proline


Succinyl CoA
MoVIe
Mehtionine,valine,Isoluecine

alcaptonuria:homogentisate
all caps:homo(dostana) gents

maple syrup:KIVi
leucine,valine,Isoluecine

Phenylketonuria:phenylalanine hydroxylase

hydroxy laces?

Saturday, November 22, 2008

u asked for it...fermentation(micro)

fermentation
EM pathway
at..bikanerwaala

homolactic:entry of bikaner waala,wahan par doodh mil raha hai.
mixed acid:ground floor k counter par,banda cocktail bana raha hai aur pooch ra hai,tezab kitna daalu?

butanediol:ice cream counter par,sunny deol(diol) ka cut out hai.acetoin:sunny deol is toying with the acid.

butyric acid and butanol acetone:paani peene ki jagah par,tyre pada hua hai,glasses ko bandhne ke liye.

propionic acid:dosa counter par,bahut saare pans pade hue hain...jo unusual se hain...


phosphoketolase:first floor par,counter par,bahut saare keet patange hain,jo ud rahe hain aur cashier ko pareshan kar rahe hain.
G3p(GAP) and acetyl phosphate...cashier ne GAP ki shirt pehni hai aur uske peeche ke menu board par,acid tiles lagi hui hain.

ED pathway:
at the loo waali jagah,first floor.KDPG..pareshaan karne waale keede(KeeDe) tumhaare peeche aa jaate hain into the loo..u run to harjyot paagi(PG) for help.

Wednesday, November 19, 2008

learn micro...the bakwass way..

dunno..but wrote this up to learn the calvin cycle and green non sulfur bacteria.

green S bacteria:

reverse T?CA cycle
citrate lyase.
all other enzymes are same.



green non S
hydroxy propionate pathway
place:LRC top floor

kone ki table->books ki lift->computers->ingraham waala rack->aunty ki seat->moveable rack->andar ka kamara->side ke rack->sheeshe ke doors

acetyl CoA
kone ki table:acid with tiles.

malonyl CoA
lift:melons

5 steps
computers:5 corresponds to a hook,u re typing ont the keyborad using a hook

propionyl CoA
rack:ye bahut ganda hai...baban oil?pappan oil?propan oil?

Methyl malonyl CoA
aunty:methanol ki min khaa ri hain..baalon mein tel laga hua hai..

Succinyl CoA
chota rack:lollipop...??

3 steps
andar ka kamra:3->heart.andar hearts rugery happens for people who get heart attacks after visiting the LRC for the first time.

Malyl CoA
bade rack:mal....ek detective ki meeting...maal kahan laana hai?centre ke do racks ke beech mein.

glyoxalate
seeshe ka darwaaza:gaai and ox bandhe hue hain tot the seeshe ka darwaaza...maaawww...







cyanobacteria and purple bacteria

RubisCo and phosphoribulokinase

place:g9 ke bahar
mitti ki jagah,jahan pohe lagte kum aur ukhaade jyaada jate hain->kopcha->g9 ka gate->regulator(jo bech mein se toota hua hai)->teacher table->projector->dosri side ke switch->doosri side ka gate->bada waala gat jo khula rehta hai,jo orange colour ka hai->fire ka cupboard


Ribulose-1,5-bisphosphate(RuBP)
(rubisco)
miiti:rubis pade hue hain wahan par,aur 1(danda) ke aage 5(hook) laga kar tum unhe doondh rahe ho.

2X 3phosphoglyceric acid
Kopcha:aape apne ghar ke guests ko wahan baithaya hai,and u r saying..dil(3) ka sharbat peejiye..

1,3 bisphosphoglycerate
gate:gate par bhabhi ji kadi hain..aap unse kehte hain,bhabhi ji aap bhi peejiye..(BPGA)

glyceraldehyde 3 phosphate
regulator:regulatoron ne ek hip hop gang banaya hai and they r wearing GAP t shirts.

dihydroxy acetone phosphate
(dhap)teacher table:teacher ko peeche se aake u say Dhappa!

fructoSe 1,6 bisphosphate
projector ke upar fruits pade hain and u r trying to bring them down using a danda..while the teacher behind you coms up with a hockey stick to take his revenge.

fructose-6-phosphate
doosri side ke switch mein tum hockey stick kmaar maar k andar chipe fruits ko bahar nikaane ki koshish kar rahe ho.

glucose
momentary relief ke liye,tum doore side ke gate par glucon d ka khaali carton chipka dete ho.

ribulose-5-phosphate
rubies se jada hua orange gate.aur uspar hook laga kar log rubis chura re hain.

Sunday, November 16, 2008

how to black mail your teachers...

Now this is really true...this is what we did plan to do when we were sick of waiting for hours(half hour) for our turn in the biotechnology experiment.

There's an apparatus called the laminar flow,just to keep the air inside it sterile.Since we have 3 such things in our college(which is still great,comparing to other colleges),we still have to wait while our turn comes up to use it.

So,the way we devised was very simple.a few phone calls,a few sim cards and peace of mind.

Step 1.
look for your teachers keys lying on the table around the lab.

Step 2.steal it.

now comes the fun part..
call up your teacher from the cold room(every biotech lab has one,in order to keep things which we have already spoit,unspoiled).Say to her in a heavy voice..

"aapka chaabi ka guccha humaare pass hai.agar gucche ki salaamati chahte ho,to chup chap group 4 ko sabse pehle experiment karne do."

teacher will reply:tumhe jo chahiye wo le lo,bas mere challe ko kuch mat karna.

you:jyaada hoshaari dikhaane ki koshish ki to tumhaari challe ke tukde tukde kar ke washing room me chupa denge.agali awaaz tumhaare challe ki hogi...

place the key ring in front of the handset and shake it..

teacher:nahiiii....

you:hmm...agali baar phone karke batayenge ki challa kahan aur kab milega.


move out of the cold room,see her reaction,see how many people are left to do the experiment.you suddenly see her talking to the lab assistant.

you call up again:hmm...tumhe kahan nahi tha jyaada hoshiyaari karne ki koshish ki to..
ab tumhaare challe se tumhaari gaadi ki chabi..

remove the key marked "maruti" from the challa.

teacher:nahiii...mere gaadi ki chaabi nahi!

you:to kyun ki itni hoshiyaari?chup chap experiment ka samaan le kar,cold storage mein aa jao.Aur is baar kissi lab assistant ya HOD ko iktila kari to...

teacher:haan haan theek hai..

By the time,she comes to the cold storage,you leave the chaabi ka challa there,move to the laminar flow,do your experiment(by this time,everyone would have done theirs,and youd've had your fun),
ask some doubts to the lab assistant(to appear innocent and completely interested),and then while your teacher is inside the cold room,lock her from outside!!

easy na!

courtesy:fankee(ankit) and words by sameer..